Online sports education for college students during covid-19 / Ensino on-line de esportes para estudantes universitários durante a covid-19 / Enseñanza en línea de deportes para universitarios durante el covid-19

ABSTRACT Introduction: There were difficulties in teaching online courses during the COVID-19 period, mainly due to the particularities of sports, requiring activities and physical training for good use. Objective: Explore the design of the online university physical education curriculum and the home exercise requirements of university students during the COVID-19 incidence. Methods: We adopted the form of questionnaires and experiments for 544 students and 5 professors volunteering for the investigation. The questionnaires were graded, and statistically analyzed the data obtained from the questionnaire. Results: The investigation shows that 40.26% of college students exercise between 60 to 90 minutes, 23.53% between 30 to 60 minutes, 21.51% between 90 to 120 minutes, and 10.48% between 0 to 30 minutes daily. Only 4.23% of college students reported exercising for more than 120 minutes daily. In addition, the impacts of exercising with online teaching are better than the procedure seen in students with independent exercise. Conclusion: Physical education teachers should adapt student-related home sports programs according to the actual needs of the local situation and equipment arrangement so that college students are not limited in their home environments, improving the teaching effect in online college physical education courses. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: Houveram dificuldades no processo de ensino de cursos on-line durante o período da COVID-19, principalmente devido às particularidades do esporte, exigindo atividades e treinamentos físicos para um bom aproveitamento. Objetivo: Explorar a concepção do currículo de educação física universitária on-line e as necessidades de exercícios em casa pelos estudantes universitários durante a incidência do COVID-19. Métodos: Adotou-se a forma de questionário e experimentos, respectivamente, para 544 estudantes e 5 professores voluntários à investigação. Os questionários foram classificados e analisou-se estatisticamente os dados obtidos a partir do questionário. Resultados: Os resultados da investigação mostram que 40,26% dos estudantes universitários se exercitam entre 60 a 90 minutos, 23,53% entre 30 a 60 minutos, 21,51% entre 90 a 120 minutos, e 10,48% entre 0 a 30 minutos diariamente. Apenas 4,23% dos estudantes universitários informaram exercitarem-se por mais de 120 minutos todos os dias. Além disso, os impactos pelo exercício com o ensino on-line são melhores do que o procedimento verificado nos estudantes com exercícios independentes. Conclusão: Os professores de educação física devem adaptar os programas de esportes domésticos relacionados aos estudantes de acordo com as necessidades reais da situação e da disposição de equipamentos locais, para que os estudantes universitários não se limitem em seus ambientes domésticos, melhorando o efeito de ensino nos cursos de educação física universitária on-line. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: Hubo dificultades en el proceso de enseñanza de los cursos en línea durante el período de COVID-19, principalmente debido a las particularidades de los deportes, que requieren actividades y entrenamiento físico para un buen uso. Objetivo: Explorar el diseño del plan de estudios de educación física universitaria en línea y las necesidades de ejercicio en casa por parte de los estudiantes universitarios durante la incidencia del COVID-19. Métodos: Se adoptó la forma de cuestionario y experimentos, respectivamente, para 544 alumnos y 5 profesores voluntarios para la investigación. Se calificaron los cuestionarios y se realizó un análisis estadístico de los datos obtenidos con el cuestionario. Resultados: Los resultados de la investigación muestran que el 40,26% de los estudiantes universitarios hace ejercicio entre 60 y 90 minutos, el 23,53% entre 30 y 60 minutos, el 21,51% entre 90 y 120 minutos y el 10,48% entre 0 y 30 minutos diarios. Sólo el 4,23% de los estudiantes universitarios declararon hacer ejercicio durante más de 120 minutos al día. Además, los impactos por el ejercicio con enseñanza en línea son mejores que el procedimiento verificado en los alumnos con ejercicios independientes. Conclusión: Los profesores de educación física deben adaptar los programas de deportes en casa de los estudiantes según las necesidades reales de la situación y la disposición local de los equipos, para que los estudiantes universitarios no se vean limitados en sus entornos domésticos, mejorando el efecto de la enseñanza en los cursos universitarios de educación física en línea. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Research progress of avermectins in anti-tumor / 国际肿瘤学杂志

Avermectins can affect biological processes of multiple tumor, including tumor cell proliferation and metastasis, cell cycle arrest, induction of apoptosis and autophagy, regulation of tumor microenvironment and tumor stem cells. Avermectins can be administered alone or combined with chemotherapeutic drugs to reverse multidrug resistance. To further explore the anti-tumor mechanism of avermectins will provide reliable experimental and theoretical guidance for future clinical application.

Effect of preoperative cognitive behavioral therapy on pain catastrophizing in patients with orthopedic trauma / 中华麻醉学杂志

Objective:To evaluate the effect of preoperative cognitive behavioral therapy (CBT) on pain catastrophizing in the patients with orthopedic trauma.Methods:A total of 120 patients with lower extremity bone trauma, aged 18-64 yr, of American Society of Anesthesiologists physical status Ⅰor Ⅱ, with body mass index of 18-28 kg/m 2, with Pain Catastrophic Scale (PCS) score on admission >16, scheduled for surgical treatment, were enrolled.The patients were divided into 2 groups ( n=60 each) by the stratified randomization method based on the type of fracture: CBT group and routine group (group R). Group CBT received CBT for pain through the internet on the day of admission and one day before operation.The patients in both groups underwent reduction and internal fixation of lower extremity fractures under combined spinal-epidural anesthesia.The PCS scores were recorded immediately after admission and on the morning of the operation day.The effective pressing times of the patient-controlled analgesia pump, consumption of analgesics for rescue analgesia, and occurrence of nausea and vomiting within 48 h after operation were recorded.The visual analogue scale score of the surgical site during activity and occurrence of the score >3 at 3 months after operation and use of opioids within 3 months after operation were recorded. Results:Compared with group R, the PCS score was significantly decreased on the morning of the operation day, the pressing times of the patient-controlled analgesia pump, consumption of analgesics for rescue analgesia and incidence of nausea and vomiting within 48 h after operation were decreased, the requirement for opioids within 3 months after operation was decreased ( P<0.05), and no significant change was found in VAS score during activity and occurrence of the score >3 at 3 months after operation in group CBT ( P>0.05). Conclusions:Preoperative CBT can reduce the degree of pain catastrophizing and is helpful in increasing the quality of postoperative analgesia in the patients with orthopedic trauma.

Quality study of Prunellae spica dispensing granules based on standard decoction / 国际中医中药杂志

Objective:To establish the quality evaluation method of Prunellae spica dispensing granules based on three quality indexes of standard decoction. Methods:Fourteen batches of Prunellae spica were collected from different habitats. According to technical requirements, fourteen batches of Prunellae spica standard decoction and three batches of formula granules were prepared and the paste-forming rates were calculated. The fingerprints of Prunellae spica standard decoction and formula granules were established by Ultra High Performance Liquid Chromatography (UPLC). The similarity values of fingerprints between dispensing granules and standard decoction were calculated. The content and transferring rate of Rosmarinic acid were determined and calculated. Results:The average paste-forming rate of Prunellae spica was (12.59±2.32)%. The paste-forming rates of the three batches were 11.14%, 10.78% and 10.39% respectively. The average content of Rosmarinic acid in standard decoction was (18.99±9.74)mg/g. The average transferring rate was (60.58±7.87)%. The contents of three batches were 7.40 mg/g, 7.49 mg/g and 7.09 mg/g. The transferring rates were 52.06%, 50.10% and 50.40% respectively. Nine common fingerprint peaks were identified in the fingerprints of standard decoction and formula granules, two of which were identified as Rosmarinic acid and Caffeic acid by comparison of reference substance. The fingerprints similarity of Prunellae spica dispensing granules and standard decoction were 0.954, 0.973 and 0.952, respectively. Conclusions:The quality indexes of three batches of formulation granules are consistent with standard decoction. This method could provide reference for the establishment of quality standard of Prunellae spica dispensing granules.

Research Progress in Epigenetics of Pancreatitis-cancer Transformation / 肿瘤防治研究

Pancreatic ductal adenocarcinoma is one of the most common malignant tumors. Its early diagnosis and treatment are very difficult, and the mortality and recurrence rate are high. Chronic pancreatitis is an important risk factor for pancreatic cancer and has great potential to develop into pancreatic cancer. Therefore, it is necessary to study the mechanism of pancreatitis-cancer transformation for early diagnosis and treatment of pancreatic cancer. In recent years, more and more studies have shown that epigenetic modification plays an important role in pancreatitis-cancer transformation, and can provide an effective target for the diagnosis and treatment of pancreatic cancer. This article summarizes the role and mechanism of epigenetics in pancreatitis-cancer transformation based on the current research progress.

The Chinese guidelines for the diagnosis and treatment of pancreatic neuroendocrine neoplasms (2020) / 中华消化外科杂志

Pancreatic neuroendocrine neoplasms (pNENs) are highly heterogeneous, and the management of pNENs patients can be intractable. To address this challenge, an expert committee was established on behalf of the Chinese Pancreatic Surgery Association, Chinese Society of Surgery, Chinese Medical Association, which consisted of surgical oncologists, gastroenterologists, medical oncologists, endocrinologists, radiologists, pathologists, and nuclear medicine specialists. By reviewing the important issues regarding the diagnosis and treatment of pNENs, the committee concluded evidence-based statements and recommendations in this article, in order to further improve the management of pNENs patients in China.

PKD3 promotes metastasis and growth of oral squamous cell carcinoma through positive feedback regulation with PD-L1 and activation of ERK-STAT1/3-EMT signalling / 国际口腔科学杂志·英文版

Oral squamous cell carcinoma (OSCC) has a high incidence of metastasis. Tumour immunotherapy targeting PD-L1 or PD-1 has been revolutionary; however, only a few patients with OSCC respond to this treatment. Therefore, it is essential to gain insights into the molecular mechanisms underlying the growth and metastasis of OSCC. In this study, we analysed the expression levels of protein kinase D3 (PKD3) and PD-L1 and their correlation with the expression of mesenchymal and epithelial markers. We found that the expression of PKD3 and PD-L1 in OSCC cells and tissues was significantly increased, which correlated positively with that of mesenchymal markers but negatively with that of epithelial markers. Silencing PKD3 significantly inhibited the growth, metastasis and invasion of OSCC cells, while its overexpression promoted these processes. Our further analyses revealed that there was positive feedback regulation between PKD3 and PD-L1, which could drive EMT of OSCC cells via the ERK/STAT1/3 pathway, thereby promoting tumour growth and metastasis. Furthermore, silencing PKD3 significantly inhibited the expression of PD-L1, and lymph node metastasis of OSCC was investigated with a mouse footpad xenograft model. Thus, our findings provide a theoretical basis for targeting PKD3 as an alternative method to block EMT for regulating PD-L1 expression and inhibiting OSCC growth and metastasis.

Moderating effect of sleep quality on the relationship between problematic mobile phone use and anxiety of college students / 中国学校卫生

Objective@#To describe the prevalence of problematic mobile phone use and anxiety in college students, and explore the mediating effect of sleep quality on the relationship between problematic mobile phone use and anxiety, and to provide reference for physical and mental health promotion of college students.@*Methods@#One medical college and a comprephensive college were selected in Hefei city of Anhui Province and Shangrao City of Jiangxi Province, respectively, and a cross-sectional survey was conducted. A total of 1 135 valid questionnaires were collected. The self-rating questionnaires regarding basic information of college students, use the Self-rating Questionnaire for Adolescent Problematic Mobile Phone Use(SQAPMPU) and the Self-Rating Questionnaire for Depression-Anxiety-Stress for Adolescent Problematic Mobile Phone Use(DASS-21) was used to evaluate problematic mobile phone use and anxiety, respectively. The Pittsburgh Sleep Quality Index(PSQI) was used to evaluate sleep quality.@*Conclusion@#The detection rates of college students with problematic mobile phone use and poor sleep quality were 24.6% and 13.3%, respectively. The detection rates of college students anxiety grouped by severity were 5.1%, 23.9%. Multiple Logistic regression analysis showed a positive linear correlation between problematic mobile phone use and anxiety[OR values(95%CI) were 1.86(1.01-3.44), 4.34(3.14-5.99), P<0.01]. The results of process showed that sleep quality played a moderating role between problematic mobile phone use and anxiety(R2=0.37, F=220.52, P<0.01). Interaction term s β=0.09(P<0.05).@*Conclusion@#The prevalence of problematic mobile phone use is high in college students, and problematic mobile phone use is positively related to anxiety. Good sleep quality can alleviate the relationship between mobile phone dependence and anxiety of college students.

Analysis of differences in intestinal microbiome in patients with psoriasis vulgaris by using 16S rRNA gene sequencing / 中华皮肤科杂志

Objective:To investigate differences in intestinal microbiome between adult patients with psoriasis vulgaris and healthy individuals.Methods:Fecal samples were collected from 22 patients with confirmed psoriasis vulgaris and 23 healthy controls in Hospital for Skin Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College from September 2017 to February 2018. The total DNA of intestinal flora was extracted and amplified, and the next-generation 16S rRNA gene-targeted sequencing was performed to analyze the diversity and distribution of intestinal flora. Species annotation and classification were performed according to Silva database, and rank sum test was used to analyze species differences among samples at different taxonomic ranks; QIIME software (Version 1.9.1) was used to calculate the number of operational taxonomic units (OTUs) and main indices of α diversity (Chao1 index, Shannon index and Simpson index) , and t test to analyze differences in indices; PCoA analysis was performed to analyze the difference in β diversity, and differences in microbial community composition structure were analyzed between the two groups by using permutational multivariate analysis of variance; rank sum test and linear discriminant analysis effect size (LEfSe) analysis were used to evaluate the species difference. Results:No significant difference in the number of OTUs was observed between the psoriasis group (147.55 ± 57.07) and healthy control group (148.96 ± 50.45, t = 0.088, P = 0.930) . In addition, there were no significant differences in the Shannon index, Chao1 index or Simpson index between the psoriasis group (4.08 ± 0.80, 169.52 ± 63.17, 0.87 ± 0.07, respectively) and healthy control group (4.11 ± 0.94, 175.36 ± 53.59, 0.86 ± 0.90, respectively; t = 0.12, 0.34, 0.27, all P > 0.05) . PCoA analysis showed that the first and second principal components explained 49.8% and 15.62%, respectively, of the total variance between the psoriasis group and healthy control group, and permutational multivariate analysis of variance revealed that the β diversity significantly differed between the two groups ( P = 0.011) . Different microbes between the psoriasis group and healthy control group included Firmicutes, Clostridia, Clostridiales, Erysipelotrichales and Erysipelotrichaceae, whose abundance significantly increased in the psoriasis group, as well as Epsilonproteobacteria, Campylobacterales, Campylobacteraceae, Campylobacter, Bacteroidales and Bacteroidaceae, whose abundance significantly increased in the healthy control group. Conclusion:The intestinal microbiome differs between patients with psoriasis vulgaris and healthy individuals, which may serve as potential biomarkers for psoriasis vulgaris.

Clinical efficacy of surgical resection after conversion therapy for advanced gastric cancer / 肿瘤研究与临床

Objective@#To observe the clinical effect of surgical resection after conversion therapy for patients with advanced gastric cancer.@*Methods@#The clinical data of 23 patients with advanced gastric cancer who were admitted to Shanxi Provincial Cancer Hospital from January 2015 to October 2017 were retrospectively analyzed. All patients underwent multi-disciplinary treatment(MDT)-directed conversion therapy, and D2 radical gastrectomy was performed after the disappearance of non-healing factors. The remission of patients after conversion therapy and the related intraoperative and postoperative indicators were analyzed.@*Results@#After the conversion therapy, there were 2 cases (8.7%) of complete remission, 13 cases (56.5%) of partial remission and 8 cases (34.8%) of stable disease. The remission rate of the conversion therapy was 65.2% (15/23). In all 23 cases, the surgical time was (135.5±32.1) min, the intraoperative blood loss was (356.7±101.3) ml, the number of lymph nodes resected was 35.5±10.3. The time of gastric tube indwelling was (70.5±29.6) h, the time to first liquid intake was (85.0±21.5) h, the time of drainage tube placement was (196.2±36.2) h and the postoperative hospital stay was (10.7±3.2) d. After surgery, two cases had grade ⅢA post-operative complications and 2 had gradeⅡ. All patients were followed up, the median progression-free survival time was 13.5 months and the median overall survival time was 21.9 months.@*Conclusion@#The survival time of patients with advanced gastric cancer can be prolonged by D2 radical gastrectomy after the MDT-guided conversion therapy.

Relationship between intestinal flora and psoriasis / 中华皮肤科杂志

Psoriasis is a chronic recurrent inflammatory skin disease,and its pathogenesis is still unclear.At present,it is believed that immune,genetic and environmental factors play important roles in the occurrence of psoriasis.More and more studies have indicated that the imbalance of intestinal flora is associated with immune diseases,especially inflammatory bowel disease.The pathogenesis and clinical manifestations of psoriasis are similar to those of inflammatory bowel disease,and psoriasis is proved to be associated with changes in intestinal microbial flora.This review discusses the association between the imbalance of intestinal flora and psoriasis.

Value of 68Ga-PSMA-11 PET/CT SUVmax combined with MRI ADC in the diagnosis of prostate cancer / 中华核医学与分子影像杂志

Objective To evaluate the combined application of the maximum standardized uptake value (SUVmax) of 68Ga-N,N'-bis (2-hydroxy-5-(carboxyethyl) benzyl) ethylenediamine-N,N'-diacetic acid (HBED-CC)-(Ahx) Lys-CO-Glu (PSMA-11) PET/CT imaging and the apparent diffusion coefficient (ADC) value of MRI in the differential diagnosis of prostate cancer (PCa).Methods Seventy untreated patients (age:(68.3± 12.1) years) with suspected PCa between December 2016 and April 2018 were prospectively studied.All patients underwent PET/CT and MRI examinations.The SUVmax and average ADC value were measured as diagnostic parameters,and the SUVmax/ADC ratio was calculated.The sensitivity and specificity of the 3 parameters were calculated by receiver operating characteristic (ROC) curve analysis.Results Of the 70 patients,31 were pathologically diagnosed as benign diseases and 39 were as PCa.The diagnostic sensitivity and specificity of average ADC value for PCa were 81％ and 72％,respectively,with a threshold value of 1.08× 10-3 mm2/s (b=1 000 s/mm2).The diagnostic sensitivity and specificity of SUVmax were 82％ and 71％ for PCa with a critical value of 7.69,and those were 72％ and 93％ with SUVmax/ADC ratio of 11.87.Conclusion The combination of 68Ga-PSMA-11 PET/CT and MRI can improve the diagnostic specificity for PCa,and the SUVmax/ADC ratio is a valuable differential diagnostic index.

Effect of Aspergillus fumigatus on the expression of tumor necrosis factor-α and activation of intracellular signaling molecule p38 mitogen-activated protein kinase by a human acute monocytic leukemia cell line THP-1 / 中华皮肤科杂志

Objective To evaluate the effect of Aspergillus fumigatus on the expression of tumor necrosis factor-α (TNF-oα) and activation of intracellular signaling molecule p38 mitogen-activated protein kinase (p38MAPK) in a human acute monocytic leukemia cell line THP-1.Methods Cultured THP-1 cells (2 x 105/ml) were divided into 4 groups to be treated with Aspergillus fumigatus suspensions at concentrations of 106 and 107 colony-forming units (CFU)/ml (106-and 107-CFU/ml Aspergillusfumigatus groups),100 mg/L β-glucan (a positive stimulus,β-glucan group),culture medium (blank control group) respectively for 1,3 and 6 hours.Real-time fluorescence-based quantitative PCR (qPCR) was conducted to determine the mRNA expression of TNF-α in the THP-1 cells in the above groups.Some other THP-1 cells were treated with 107 CFU/ml Aspergillusfumigatus suspensions (107-CFU/ml Aspergillusfumigatus group),β-glucan (β-glucan group) and culture medium (blank control group) separately for 24 hours,and enzymelinked immunosorbent assay (ELISA) was performed to detect the level of TNF-α in the culture supernatant of THP-1 cells.Western blot analysis was conducted to detect the levels of p38MAPK and phosphorylated p38MAPK in THP-1 cells after 15-,30-and 60-minute treatment with 107 CFU/ml Aspergillusfumigatus suspensions.After 2-hour incubation with the p38MAPK inhibitor SB203580 (20 μmol/L),some THP-1 cells were additionally treated with 107 CFU/ml Aspergillus fumigatus suspensions,β-glucan and culture medium separately for 6 hours,and those without SB203580 treatment served as the control group.Then,qPCR was performed to measure the mRNA expression of TNF-α in the THP-1 cells in the above groups.Results The mRNA expression of TNF-α significantly differed among the 106-and 107-CFU/ml Aspergillus fumigatus groups,β-glucan group and blank control group (F =110.983,P ＜ 0.001),and significantly increased over time (F =701.680,P ＜ 0.001).After 24-hour treatment with 107 CFU/ml Aspergillus fumigatus suspensions,the TNF-α level(6 236.30 ± 437.12 ng/L)significantly increased compared with the blank control group (132.10 ± 0.61 ng/L,P ＜ 0.01).Thirty minutes after the treatment with 107 CFU/ml Aspergillusfumigatus suspensions,the phosphorylated p38MAPK level significantly increased,but started to decrease at 60 minutes.The mRNA expression of TNF-α was significantly lower in the SB203580-treated Aspergillusfumigatus groups (3.83 ± 0.62) than in the SB203580-untreated Aspergillus fumigatus groups (187.23 ± 21.62).Conclusion After the treatment with Aspergillus fumigatus,human THP-1 cells can activate the signal molecule p38MAPK and secrete TNF-α,suggesting that monocytes may participate in the innate immune response to Aspergillusfumigatus infection.

Progress and reflection on the therapeutic strategy of targeting the pancreatic cancer microenvironment / 中国肿瘤临床

Pancreatic cancer(PC)is a highly malignant disease.Significant fibrosis and tumor heterogeneity are important characteris-tics of PC that have been attributed to the poor chemotherapeutic response and poor prognosis of patients with PC.Therefore,it is im-perative to develop more effective treatment strategies to improve the survival rate of PC patients.In order to improve the response rates and accuracy of treatment for PC,domestic and foreign scholars have directed their efforts in elucidating the mechanisms of tu-mor microenvironment that mediate PC development to identify more effective treatment strategies targeting this microenvironment. This paper reviews the latest advances and therapeutic strategies for targeting the tumor microenvironment,under the concept of pre-cise treatment,and also provides a new idea for further improving the survival rates of patients with PC.

Effects of intrathecal injection of SOCS3 on pain behavior and expression of proinflammatory cyto-kines in mice with neuropathic pain / 中华行为医学与脑科学杂志

Objective To evaluate the role of suppressor of cytokine signaling 3 ( SOCS3) in the spinal cord of chronic sciatic constriction injury ( CCI) mice. Methods Part one:four Kunming mice were selected to receive the CCI operation by sciatic nerve ligation. Seven days after operation the mice were sac-rificed and L4～6 segments of the spinal cord were removed. The co-expression of SOCS3 with NeuN ( for neurons),GFAP (for astrocytes),or IBA1 (for microglia) in spinal were detected by immunohistofluores-cence. Part two:thirty-two Kunming mice were divided into 4 groups according to random number table:sham operation group (group SH),CCI group (group BP),CCI+Lenti-SOCS3 group (group BS),CCI +Lenti-vector group (group BV). Group BS were intrathcal injected of Lenti-SOCS3 (2 μl) and group BV were intrathcal injected of Lenti-vector (2 μl) on 7 d,8 d,9 d after operation. Paw withdrawal latency ( PWL) and paw withdrawal threshold ( PWT) were measured at 1 d before operation and 5,7,10,12,14 d after operation. Mice were then sacrificed and L4～6 segments of the spinal cord were removed for determina-tion of GFAP,IBA-1 by Western blot and IL-6,IL-1β,TNF-α by Elisa on 14 d. Results SOCS3 was dis-tributed in dorsal horn,and expressed in astrocytes and microglia,but hardly colocalized with neurons. Com-pared with group SH,the PWL and PWT of group BP and BV were significantly decreased after operation (all P<0. 05),and the expression of GFAP,IBA-1,IL-6,IL-1β and TNF-α was significantly increased (all P<0. 05). Compared with group BV,the PWL (5. 1±0. 9,7. 5±0. 8,7. 2±1. 4) and PWT (6. 1±1. 4,8. 9± 1. 1,8. 2±2. 1) of group BS was significantly increased on 10,12,14 d (all P<0. 05),and the expression of GFAP (1. 69±0. 45),IBA-1(1. 76±0. 25),IL-6 (181±8),IL-1β (151±7),TNF-α (216±9) was signifi-cantly downregulated (P<0. 05) . Conclusion SOCS3 alleviates neuropathic pain by inhibiting the glial ac-tivation and the expression of proinflammatory cytokines IL-6,IL-1β,TNF-α.

Comparison of compression stability in left common iliac vein at different time by contrast-enhanced CT / 中南大学学报(医学版)

Objective:To explore the compression stability in left common iliac vein (LCIV) at different times by contrast-enhanced CT in the same patients.Methods:This retrospective study included 124 patients (without signs of lower extremity venous diseases),who underwent contrast-enhanced CT at pelvic cavity and reexamined within one year.The degree of compression changes in LCIV was calculated and compared between the two examinations.Moreover,the relationship between age and the compression rate of LCIV was analyzed.Results:In the two examinations for the same patient,the compression rate of LCIV was almost the same,and there was no significant difference (t=-0.957,P＞0.05).Moreover,the compression rate of LCIV did not significantly change between different gender (P＞0.05).No matter whether the compression rate was more than 50％,there was no significant difference in the rate of LCIV compression between two examinations (P＞0.05).There was no significant correlation between age and the compression rate of LCIV (age＜50 years:r=0.152,P＞0.05;age ≥ 50 years:r=-0.214,P＞0.05).Conclusion:The compression degree of LCIV is almost stable,which is not significantly correlated with the age.The contrast-enhanced CT for single time can accurately display and diagnose the compression degree of LCIV.

High-frequency ultrasound blood flow imaging based on laser speckle algorithm / 国际生物医学工程杂志

Objective To study the application of laser speckle contrast algorithm in high-frequency ultrasound blood flow imaging. Methods The laser speckle contrast algorithm was extended to the field of high-frequency ultrasound blood flow imaging. The high-frequency ultrasound structure images were processed by using the classic laser speckle temporal contrast algorithm to suppress the static tissue signals, enhance the dynamic blood flow signals, and obtain the high-frequency ultrasound blood flow images. The simulation experiments on flow phantoms were conducted to verify the effectiveness of the algorithm, and then human skin tissue in vivo experiments were performed to verify the applicability of the algorithm. The performances of blood flow images which obtained from ultrasound structure images with different frames by laser speckle contrast algorithm were compared and analyzed. Results The blood flow information in a tube was clearly visible in the flow phantoms experiments. The static skin tissue signals were significantly suppressed, dynamic blood flow signals were strengthened in the human skin tissue in vivo experiments, and high-frequency ultrasound blood flow images were obtained. By comparing and analyzing the performances of blood flow images, the results showed that the best blood flow images could be obtained when the number of frame was set to 10 frames. Conclusions The speckle contrast algorithm is feasible and has good application prospects in high-frequency ultrasound blood flow imaging.

Analysis of ADAR1 gene mutation in a pedigree affected with dyschromatosis symmetrical hereditaria / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To detect mutation of adenosine deaminase acting on RNA1 (ADAR1) gene in a pedigree affected with dyschromatosis symmetrical hereditaria (DSH).</p><p><b>METHODS</b>Clinical data and peripheral blood samples of the patients from the pedigree were collected. Potential mutations of the ADAR1 gene were screened among 2 patients, 2 unaffected individual from the pedigree as well as 50 unrelated healthy controls by PCR amplification and direct sequencing.</p><p><b>RESULTS</b>A c.3463C>T (p.R1155W) missense mutation of the ADAR gene was identified in the 2 patients, which was absent in the 2 healthy relatives and 50 unrelated controls. The mutation has been previously identified among 5 Chinese families and was the most common mutation site.</p><p><b>CONCLUSION</b>The c.3463C>T missense mutation of the ADAR gene probably underlies the disease in this pedigree.</p>

Clinical value of magnetic resonance venography in diagnosing Cockett syndrome / 介入放射学杂志

Objective To evaluate non-enhancement magnetic resonance venography (MRV) of iliac vein in diagnosing Cockett syndrome.Methods Magnetic resonance iliac venography was performed with Ingenia 3.0 T superconducting type MRI system.Abdominal surface coil was employed.The scanning sequences included M2DIPEAR (TR/TE=45/5.8 ms,flip angle=60°),THRIVE (TR/TE=6.8/3.5 ms,flip angle=10°),BTFESPAIR (TR/TE=3.4/1.7 ms,flip angle=80°) and FLAIR (TR/TE=9 000/120 ms,flip angle 90°).The layer thickness of 3 mm was used in all scanning,and the average number of acquisition was 3 times.Results On MRV imaging,Cockett syndrome was characterized by narrowed anteroposterior diameter and broadened transverse diameter of the compressed iliac vein,and curved impression could be seen on its anterior border,and collateral vessel formation could be observed.The mean diameters of the left iliac veins in the light,mnedium and severe patients with Cockett syndrome were 7.52,4.83 and 2.76 mm respectively,with the average compression ratios being 37％,69％ and 83％ respectively.Conclusion Non-enhanced MRV is a feasible method for the diagnosis of iliac vein stenosis,this examination is especially suitable for the checking needs of specific population.

The application of combined venography in the diagnosis of iliac vein compression syndrome with dual-source CT / 实用放射学杂志

Objective To explore the clinical value of the combined dual-source CT venography(CTV)for the diagnosis of iliac vein compression syndrome(IVCS).Methods 43 patients with clinically suspected IVCS underwent CTV examination.Among them,22 cases were assessed with combined CTV while 21 cases were given conventionally indirect CTV.The lower extremity deep vein was scanned from foot to head.The dual-energy technology was applied for data acquisition.Results Two kinds of CTV methods could be used directly for diagnosing IVCS and checking the asymptomatic mild iliac vein compression.32 IVCS patients were diagnosed by CTV.In 27 cases with deep venous thrombosis(DVT),the average diameter of the left iliac vein was(3.23 ± 1.45)mm and the average compression rate was 67%.The differences were statistically significant in comparisons of the average diameter of left iliac vein between IVCS patients with or without DVT and patients without IVCS.The image quality scores of bidirectional CTV were higher than indirect CTV with statistically significant difference.Conclusion Combined CTV is a feasible technique for lower extremity venography.The image quality of combined CTV is better than conventional indirect CTV with greater clinical value.